登革熱國際期刊學術論文免費下載(part I) 人氣: 543
2015-10-28
國內登革熱疫情不降反升,國際學術論文平台Elsevier(愛思唯爾)為協助疫情控制及病患治療,特別於全球醫藥新知網站製作專頁醫學新訊-登革熱-最新臨床報告,持續至12月31日,提供登革熱學術論文免費下載,以利共同對抗登革熱並有效控制疫情。

登革熱診斷
Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus
Diagnostic Microbiology and Infectious Disease 83 (2015) 30-36
During dengue outbreaks, acute diagnosis at the patient's point of need followed by appropriate supportive therapy reduces morbidity and mortality. To facilitate needed diagnosis, we developed and optimized a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that detects all 4 serotypes of dengue virus (DENV). We used a quencher to reduce nonspecific amplification. The assay does not require expensive thermocyclers, utilizing a simple water bath to maintain the reaction at 63 °C. Results can be visualized using UV fluorescence, handheld readers, or lateral flow immunochromatographic tests. We report a sensitivity of 86.3% (95% confidence interval [CI], 72.7–94.8%) and specificity of 93.0% (95% CI, 83.0–98.1%) using a panel of clinical specimens characterized by DENV quantitative reverse transcription–polymerase chain reaction. This pan-serotype DENV RT-LAMP can be adapted to field-expedient formats where it can provide actionable diagnosis near the patient's point of need.

Development and clinical evaluation of a highly accurate dengue NS1 rapid test: from the preparation of a soluble NS1 antigen to the construction of an RDT
Diagnostic Microbiology & Infectious Disease, 2015-06-01, Volume 82, Issue 2, Pages 128-134
Early diagnosis of dengue virus (DENV) is important. There are numerous products on the market claiming to detect DENV NS1, but these are not always reliable. In this study, a highly sensitive and accurate rapid diagnostic test (RDT) was developed using anti-dengue NS1 monoclonal antibodies. A recombinant NS1 protein was produced with high antigenicity and purity. Monoclonal antibodies were raised against this purified NS1 antigen. The RDT was constructed using a capturing (4A6A10, K d = 7.512 ± 0.419 × 10 −9 ) and a conjugating antibody (3E12E6, K d = 7.032 ± 0.322 × 10 −9 ). The diagnostic performance was evaluated with NS1-positive clinical samples collected from various dengue endemic countries and compared to SD BioLine Dengue NS1 Ag kit. The constructed RDT exhibited higher sensitivity (92.9%) with more obvious diagnostic performance than the commercial kit (83.3%). The specificity of constructed RDT was 100%. The constructed RDT could offer a reliable point-of-care testing tool for the early detection of dengue infections in remote areas and contribute to the control of dengue-related diseases.

Nosocomial transmission of dengue fever via needlestick. An occupational risk
Travel Medicine and Infectious Disease, 2015-05-01, Volume 13, Issue 3, Pages 271-273
Dengue fever is currently considered to be the most important arboviral cause of disease in humans, with a dramatic increase in incidence over the last 30 years. The dengue virus is a flavivirus with four antigenically distinct serotypes and is endemic in over 100 different countries in the Americas, Africa, Eastern Mediterranean, Western Pacific and Southeast Asia. This is a case report of the first documented case of dengue transmission via needlestick in the United Kingdom. Our case is of a 48 year old healthcare worker who sustained a needlestick injury from a patient with confirmed dengue fever and subsequently developed the illness himself.

The early clinical features and predictors of severe dengue and dengue shock syndrome in adult patients
Journal of Microbiology, Immunology and Infection, 2015-04-01, Volume 48, Issue 2, Pages S119-S119
A total of 1063 patients were included in the study. Mean length of illness from onset illness to hospital presentation of the 55 SD (mean age, 63 years) patients, and 1008 non-SD (mean age, 48.7 years) patients was 3.8 days and 3.7 days, respectively. Of the 55 SD patients, the mean time interval from illness onset to development of SD was 5.3 days, and from hospital presentation to occurrence of SD was 1.5 days. Three commonest warning signs at arrival were abdominal pain (45.5%), vomiting (36.4%) and pleural effusion (32%). Leukocytosis (white cell count >10×10 9 cells/L) was seen in 10 (18.2%) of 55 SD patients upon their arrival. DSS occurred in 23 (mean age, 62.4 years) (41.8%) of 55 SD patients. The mean interval from illness onset to DSS was 5.5 days. Overall, there were twelve patients died. Multivariate analysis showed elderly (adjusted odds ratio [aOR] 1.052), gastrointestinal bleeding (aOR 12.127), leukocytosis (aOR 49.744), and severe thrombocytopenia (platelet count <50×109 cells/L) (aOR 3.212) were independent predictors of SD. Predictors of DSS were aging (aOR 1.039), gastrointestinal bleeding (aOR 38.841), and severe thrombocytopenia (aOR 2.850). 

登革熱治療
Strategy in managing anticoagulation therapy following prosthetic heart valve replacement in a patient with dengue fever
International Journal of Cardiology 199 (2015) 432–434
More than 2.5 billion people (40% of the world's population) are at risk of contracting dengue fever in the tropics and subtropics. Although dengue usually presents as a self-limiting viral fever, a small proportion of patients experience a severe form that is characterized by severe plasma leakage, organ impairment, and hemorrhagic manifestations with greater mortality rates. The risk of severe bleeding is greater in older adults and in children, in those with comorbidities such as diabetes and hypertension, and in those taking non-steroidal anti-inflammatory drugs (NSAID) or anticoagulants. The aging population and the greater risk of severe dengue in the elderly increasingly raise the clinical dilemma of having to balance the risk of bleeding with that of thromboembolic events in dengue-infected patients on mandatory anticoagulant therapy.

Decreased plasma levels of the endothelial protective sphingosine-1-phosphate are associated with dengue-induced plasma leakage.
Published October 1, 2015.  Volume71, Issue4; Pages480-7
Decreased plasma S1P levels during dengue are associated with plasma leakage. Authors speculate that decreased levels of ApoM underlies the lower S1P levels. Modulation of S1P levels and its receptors may be a novel therapeutic intervention to prevent plasma leakage in dengue.

Dengue infection associated hemophagocytic syndrome: Therapeutic interventions and outcome
Journal of Clinical Virology 69 (2015) 91–95
Infection associated hemophagocytic syndrome is increasingly recognized as a potentially fatal complication of dengue fever. It should be suspected with prolonged fever beyond seven days associated with hepatosplenomegaly, hyperferritinemia, worsening cytopenias and development of multiorgan dysfunction. Surge of similar pro-inflammatory cytokines observed in dengue associated hemophagocytic syndrome and multiorgan dysfunction may indicate they are part of related inflammatory spectrum. A proportion of patients recovered with supportive therapy, however most required interventions with corticosteroids, intravenous immunoglobulin or chemotherapy. We report three cases of dengue associated IAHS with good outcome following early recognition and treatment with dexamethasone and intravenous immunoglobulin.
 
Antibody avidity following secondary dengue virus type 2 infection across a range of disease severity
Journal of Clinical Virology, 2015-08-01, Volume 69, Pages 63-67
The data show a significant increase in avidity from acute to convalescent phase followed by a decrease from convalescent phase to 3 months post-symptom onset, then a plateau. Linear regression analysis comparing antibody avidity between disease severity groups over time indicate that individuals with more severe disease (DHF/DSS) experienced greater decay in antibody avidity over time compared to less severe disease (DF), and ROC curve analysis showed that at 18 months post-illness, lower avidity was associated with previously having experienced more severe disease. These data suggest that increased dengue disease severity is associated with lower antibody avidity at later time-points post-illness.

An unusual case of dengue infection presenting with hypokalemic paralysis with hypomagnesemia
Journal of Clinical Virology, 2015-08-01, Volume 69, Pages 197-199
Neurological manifestations are unusual in dengue fever and can be due to neurotropic effect, systemic complications of dengue infection, or immune mediated. Acute hypokalemic paralysis is a rare systemic complication of dengue infection; however, hypokalemia along with hypomagnesemia has not been reported earlier. We herein report an extremely unusual and probably the first case of dengue infection in a 30-year-old male who presented to us with hypokalemic paralysis along with hypomagnesemia. This case report highlights that hypomagnesemia may be a significant complication in dengue infection. Correction of hypomagnesemia is of paramount importance to avoid refractory hypokalemia leading to severe consequences. 



Elsevier
全球醫藥新知網站
10449台北市中山北路二段96號嘉新大樓第二大樓8F N-818室
Rm. N-818, 8F, Chia Hsin Building II, NO. 96, Zhong Shan N.Road, Sec. 2, Taipei, 10449 Taiwan.
Tel: +886-2-2522-5900
Fax: +886-2-2522-1885
Email: globalmednews.elsevier@gmail.com
© 2015 ELSEVIER All rights reserved

 全球醫藥新知網站使用條款與條件

 瀏覽人數: 2209519  人

頂控科技網頁設計公司 | 嚮應式網頁布局 | 網站製作